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耶拿-Fastblot B43/B44型半干法电转印仪

analytikjena Fastblot B43/B44 Semi-dry Electro-blotting Apparatus

Fastblot B43/B44型半干法电转印仪(Semi-dry Electro-blotting Apparatus)

  For rapid semi-dry blotting of proteins up to 150 KDa Fastblot B43/B44
  • Maintenance-free metal electrodes
  • 16 x 20 cm blotting area
  • Cooling option (Fastblot B43)
Electro-blotting is an important electrophoresis method to transfer mainly proteins from polyacrylamide gels on to nitrocellulose or other carrier membranes. Semi-dry blotting is done between two horizontal plate electrodes and offers fast as well as homogeneous transfer. In contrast to tank blotting only little transfer buffer is required and transfer times are shorter. Additionally, with semi-dry blotting discontinuous buffer systems can be used to gently blot smaller proteins or to transfer protein mixtures of very different sizes more evenly.
With Fastblot B43 and B44 Biometra offers corrosion-free metal electrodes without pores. These electrodes consist of a platinum-coated titanium anode and a stainless steel cathode. The metal electrodes are easy to decontaminate and can be used for higher currents so that blotting times are reduced to 10 to 30 minutes.
Large proteins (> 100 kDa) require longer transfer times. The heat that is generated by the extended transfer can be removed using the flow-through cooling system which is available with model B43. By applying higher current (up to 5 mA/cm2 gel), proteins with higher molecular weights can be blotted faster and more quantitatively. Even smaller proteins can be transferred faster from thick gels and gels with small pores when blotting with high current.
Nucleic acids can also be electro-blotted using the Fastblot systems. However, vacuum blotting is the method of choice for nucleic acids.

Fastblot B43/B44型半干法电转印仪的订货信息

 

产品编号

产品描述

015-100 Fastblot B43, complete system, anode: platinum-coated titanium, cathode: stainless steel, electrode surface 16 x 20 cm, with flow-through cooling, incl. manual
015-200 Fastblot B44, complete system, anode: platinum-coated titanium, cathode: stainless steel, electrode surface 16 x 20 cm, without cooling, incl. manual
 
10 426 981 Whatman GB005, 200 x 200 mm, 1.2 mm thick, 25/pkg
10 426 994 Whatman GB005, 580 x 580 mm, 1.2 mm thick, 25/pkg
3030931 Whatman 3MM Chr, 580 x 680 mm, 0.34 mm thick 100/pkg
3017915 Whatman 17 Chr, 460 x 570 mm, 0.92 mm thick 25/pkg
   
上次更新时间:2016年04月28日
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