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德国耶拿公司目录

基因扩增仪(PCR)
实时荧光定量PCR仪
生物分析系统
核酸提取纯化系统
样品处理系统
恒温振荡孵育器
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适配附件和消耗品
PCR-酶/试剂/引物
  标准PCR试剂
快速PCR试剂
定量PCR试剂
酶/试剂/添加物
耶拿-分析和诊断试剂

相关产品链接

微量核酸/蛋白分析仪
 

德国耶拿-PCR标准试剂盒

Analytikjena Reagents for Standard PCR

Here you find our product range for PCR reagents which are used when performing a standard PCR. We are offering a wide range on polymerases, ladders, dNTPs and as well RT-enzymes.

01 innuTaq DNA Polymerase 02 innuTaq RED DNA Polymerase
03 innuTaq HOT-A DNA Polymerase 04 innuTaq Long Range DNA Polymerase
05 innuTaq TITAN DNA Polymerase 06 innuTaq UltraPure DNA Polymerase
07 PCR ready mix 08 PCR ready mix plus
09 innuMIX Green PCR MasterMix 10  

innuTaq HOT-A DNA Polymerase

  innuTaq HOT-A DNA Polymerase provides improved specificity and sensitivity when amplifying low-copy-number targets in complex backgrounds or when prolonged room temperature set up is required. The polymerase activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup.The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5‘ - 3‘ direction in the presence of magnesium. It also possesses a 5‘ - 3‘ polymerization-dependent exonuclease replacement activity but lacks a 3‘ - 5‘ exonuclease activity. innuTaq HOT-A DNA Polymerase requires no prolonged heating or denaturing step. The polymerase inhibiting ligand is quickly released at the increased temperature of thermal cycling.
Concentration*: 5 U/µl
Enzyme storage buffer: 20 mM Tris-HCl, 100 mM KCl, 0.1 EDTA, 1 mM DTT, 0.5% Tween-20, 0.5% Nonidet P-40, 50% (v/v) Glycerol, pH 8.0 (25°C)
PCR Buffer: 10x Hotstart Buffer complete: 200 mM Tris-HCL, 500 mM KCl, 15 mM, MgCl2, pH8,5,
10x Hotstart Buffer without MgCl2: 200 mM Tris-HCL, 500 mM KCl, pH8,5
Mg2+ solution: MgCl2 stock solution, 25 mM
Store: at –20°C, avoid frequent thawing and freezing

产品编号

产品描述

845-EZ-3000500 innuTaq HOT-A DNA Polymerase,500 units
*One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTPs into a polynucleotide fraction in 30 minutes at 70°C.
   
上次更新时间:2014年07月14日
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